Oligosaccharide analysis by mass spectrometry: a review of recent developments.

Developments Muchena J. Kailemia,† L. Renee Ruhaak,‡ Carlito B. Lebrilla,‡ and I. Jonathan Amster*,† †Department of Chemistry, University of Georgia, Athens, Georgia 30602, United States ‡Department of Chemistry, University of California at Davis, Davis, California 95616, United States ■ CONTENTS Ionization A MALDI Analysis B ESI Analysis B Ion Activation B Low-Energy Fragmentation C Electron Aided Fragmentation D MS/MS of GAGs D Photodissociation E MS/MS of Anion Complexes E N-Linked and O-Linked Oligosaccharides E Quantification F Label-Free Quantitation F Isotopic Labeling Approaches G Separations H Reversed Phase Separations H HILIC Separations I Graphitized Carbon Separations J Ion Mobility J Spatial Dispersion IM J Time Dispersion IM K Software L Conclusions M Author Information N Corresponding Author N Notes N Biographies N Acknowledgments N References N